Histopathology is one of the main methods used for diagnosis of disease. Following a medical screening process, a patient can undergo a biopsy, in which a piece of tissue is removed for further inspection and diagnostic analysis. This tissue specimen is then sliced into thin sections that are on the order of a few millionths of a meter in thickness. These thin sections of tissue contain at the microscopic scale the diagnostic information regarding the patient's condition. However, they exhibit almost no contrast under standard light microscopy. To reveal these microscopic features embedded inside tissue and bring visible contrast for inspection by a pathologist, various tissue staining methods have been created in pathology dating back to more than 150 years ago. These tissue staining procedures use different types of colored dyes that specifically label micro-scale structures in tissue, forming colorful images of specimens, which have been widely used as a gold standard diagnostic method in modern medicine.
* This article was originally published here
Sunday 10 March 2019
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